Cell Walls | wasteneyslab

Cell Wall Formation

Cellulose microfibrils are essential components of plant cell walls, providing structural support and guiding cell expansion. The organized arrangement of these microfibrils determines both the direction and extent of cell growth. Our lab is captivated by the role that microtubules play in orchestrating cellulose biosynthesis and ensuring the quality of cell wall formation.

Cryo-planed Arabidopsis stem Cryo-planed Arabidopsis stem was used to remove cytoplasmic content and to view the innermost layer of the cell wall. Scale bar = 150 um. Image by Miki Fujita.	Cryo-planed epidermis of Arabidopsis stem 50 um The top region of inflorescence stems contain recently devided cells and we often observe the cell plate. Scale bar = 50 um. Image by Miki Fujita.
Cellulose microfibrils The most recently deposited cellulose microfibrils are aligned perpendicular to the cell growth axis. Scale bar = 200 nm. Image by Miki Fujita.	Near-TIRF image of cortical microtub Cellulose-synthase-complexes (CSCs) at plasma membrane move along the cortical microtubules aligned perpendicular to the cell growth axis. Scale bar = 5 um. Image by Miki Fujita.
Golgi localization of cellulose-synthase-complexes in rsw1-1 mutant at restrictive temperature Donut-shaped structure are fluorescently-labelled cellulose-synthase-complexes (CSCs) localized in Golgi. At restrictive temperature, CSCs are absent from plasma membrane in the rsw1-1 (CesA1) mutant. Scale bar = 5 um. Image by Miki Fujita.

Cell Wall Formation

Cryo-planed Arabidopsis stem

Cryo-planed Arabidopsis stem was used to remove cytoplasmic content and to view the innermost layer of the cell wall. Scale bar = 150 um. Image by Miki Fujita.

Cryo-planed epidermis of Arabidopsis stem 50 um

The top region of inflorescence stems contain recently devided cells and we often observe the cell plate. Scale bar = 50 um. Image by Miki Fujita.

Cellulose microfibrils

The most recently deposited cellulose microfibrils are aligned perpendicular to the cell growth axis. Scale bar = 200 nm. Image by Miki Fujita.

Near-TIRF image of cortical microtub

Cellulose-synthase-complexes (CSCs) at plasma membrane move along the cortical microtubules aligned perpendicular to the cell growth axis. Scale bar = 5 um. Image by Miki Fujita.

Golgi localization of cellulose-synthase-complexes in rsw1-1 mutant at restrictive temperature

Donut-shaped structure are fluorescently-labelled cellulose-synthase-complexes (CSCs) localized in Golgi. At restrictive temperature, CSCs are absent from plasma membrane in the rsw1-1 (CesA1) mutant. Scale bar = 5 um. Image by Miki Fujita.